HEPES CAS 7365-45-9
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HEPES CAS 7365-45-9

HEPES CAS 7365-45-9 biological buffer; Reaction buffer, pre hybridization buffer and hybridization buffer for separating and analyzing RNA nuclear components; For RNA and t4rna.

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Product Description

Shandong Believe Chemical Pte. Ltd. HEPES CAS 7365-45-9 is an important hydrogen ion buffer. 

It has a good buffering capacity in the range of pH 6.8-8.2, and can control a constant chemical Book pH for a long time. When the concentration is 10-50mmol / L, the general culture medium containing 20mmol / L 4-hydroxyethyl piperazine ethanesulfonic acid can have good buffering capacity and has no toxic effect on cells.

Preparation method:

 directly use 1,2-dichloroethane as solvent, add hydroxyethyl piperazine (5.00g, 0.02mol), potassium carbonate K2CO3 (6.00g, 0.04mol), 50ml1,2-chemicalbook dichloroethane into a 100ml three mouth bottle equipped with mechanical stirring and thermometer, heat the oil bath at 90 ° C (boiling point of 1,2-dichloroethane: 85 ° C), and stir for 20h. 

The reaction was stopped, filtered, and the filtered salt was washed with 200 ml of ethyl acetate (EA). The filtrate was spin dried to obtain 2.6 g of HEPES (4-hydroxyethyl piperazine ethanesulfonic acid) as a solid.


HEPESChemicalSpecification 


 

Product Name

 

HEPES

 

Formula

 

C8H18N2O4S

 

Molecular Weight

 

238.3

 

CAS NO.

 

7365-45-9

 

Quantity

 

500KG


Items

Specification

Appearance

White crystal powder

Assay

≥99.5%

    Melting point

234-238 °C

Moisture

≤0.5%

结论

CONCLUSION

The result conform with standard




HEPES CAS 7365-45-9

Synonym:

CAS: 607-91-0

Molecular formula: C8H18N2O4S

Molecular weight:238.3

EINECS:230-907-9

Density:1.07 g/mL at 20 °C

Storage conditions: 2-8°C

PH:7.0 - 7.6

Storage conditions:Store below +30°C.

Shelf life:2 years

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