HEPES CAS 7365-45-9 biological buffer; Reaction buffer, pre hybridization buffer and hybridization buffer for separating and analyzing RNA nuclear components; For RNA and t4rna.
Shandong Believe Chemical Pte. Ltd. HEPES CAS 7365-45-9 is an important hydrogen ion buffer.
It has a good buffering capacity in the range of pH 6.8-8.2, and can control a constant chemical Book pH for a long time. When the concentration is 10-50mmol / L, the general culture medium containing 20mmol / L 4-hydroxyethyl piperazine ethanesulfonic acid can have good buffering capacity and has no toxic effect on cells.
Preparation method:
directly use 1,2-dichloroethane as solvent, add hydroxyethyl piperazine (5.00g, 0.02mol), potassium carbonate K2CO3 (6.00g, 0.04mol), 50ml1,2-chemicalbook dichloroethane into a 100ml three mouth bottle equipped with mechanical stirring and thermometer, heat the oil bath at 90 ° C (boiling point of 1,2-dichloroethane: 85 ° C), and stir for 20h.
The reaction was stopped, filtered, and the filtered salt was washed with 200 ml of ethyl acetate (EA). The filtrate was spin dried to obtain 2.6 g of HEPES (4-hydroxyethyl piperazine ethanesulfonic acid) as a solid.
HEPESChemicalSpecification
Product Name |
HEPES |
||
Formula |
C8H18N2O4S |
Molecular Weight |
238.3 |
CAS NO. |
7365-45-9 |
Quantity |
500KG |
Items |
Specification |
|
Appearance |
White crystal powder |
|
Assay |
≥99.5% |
|
Melting point |
234-238 °C |
|
Moisture |
≤0.5% |
|
结论 CONCLUSION |
The result conform with standard |
HEPES CAS 7365-45-9
Synonym:
CAS: 607-91-0
Molecular formula: C8H18N2O4S
Molecular weight:238.3
EINECS:230-907-9
Density:1.07 g/mL at 20 °C
Storage conditions: 2-8°C
PH:7.0 - 7.6
Storage conditions:Store below +30°C.
Shelf life:2 years